rfhealthЗдравоохранение Российской ФедерацииHealth care of the Russian Federation0044-197X2412-0723Federal Scientific Center of Hygiene named after F.F. Erisman10.46563/0044-197X-2020-64-6-351-357rfhealth-237Research ArticleТОКСИКОЛОГИЯ (ПРОФИЛАКТИЧЕСКАЯ, КЛИНИЧЕСКАЯ, ЭКОЛОГИЧЕСКАЯ)TOXICOLOGY (PREVENTIVE, CLINICAL, ECOLOGICAL)Окислительное карбонилирование белков ткани печени под воздействием пестицида на основе глифосата в субхроническом экспериментеOxidative carbonilation of liver tissue proteins under the influence of pesticide based on glyphosate in a subchronic experimenthttps://orcid.org/0000-0001-5648-6669МирошниковаДарья ИгоревнаMiroshnikovaDarya I.

Аспирант кафедры профильных гигиенических дисциплин с курсом гигиены, эпидемиологии и организации госсанэпидслужбы ФДПО ФГБОУ ВО РязГМУ Минздрава России, 390026, Рязань.

e-mail: d.i.miroshnikova9@gmail.com

MD, PhD-student of the Department of profile hygiene disciplines with a course of hygiene, epidemiology and organization of the state sanitary and epidemiological service, Ryazan State Medical University, Ryazan, 390026, Russia.

e-mail: d.i.miroshnikova9@gmail.com

d.i.miroshnikova9@gmail.comhttps://orcid.org/0000-0002-9959-6507РакитскийВ. Н.RakitskiiValerii N.noemail@neicon.ruhttps://orcid.org/0000-0001-5550-0625ФоминаМ. А.FominaMaria A.noemail@neicon.ruhttps://orcid.org/0000-0002-1258-9807КирюшинВ. А.KiryushinValerii A.noemail@neicon.ruhttps://orcid.org/0000-0003-0316-5479МоталоваТ. В.MotalovaTatiana V.noemail@neicon.ruФГБОУ ВО «Рязанский государственный медицинский университет имени академика И.П. Павлова» Минздрава РоссииRyazan State Medical UniversityФБУН «Федеральный научный центр гигиены имени Ф.Ф. Эрисмана» РоспотребнадзораF.F. Erisman Federal Scientific Center for Hygiene of the Federal Service for Supervision in Protection of the Rights of Consumer and Man WellbeingФГАОУ ВО «Российский национальный исследовательский медицинский университет имени Н.И. Пирогова» Минздрава РоссииPirogov Russian National Research Medical University202001012021646351357Copyright © Мирошникова Д.И., Ракитский В.Н., Фомина М.А., Кирюшин В.А., Моталова Т.В., 20212021Мирошникова Д.И., Ракитский В.Н., Фомина М.А., Кирюшин В.А., Моталова Т.В.Miroshnikova D.I., Rakitskii V.N., Fomina M.A., Kiryushin V.A., Motalova T.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.rfhealth.ru/jour/article/view/237Введение

Введение. В настоящее время изучаются патогенетические механизмы действия на организм широко применяемых гербицидов на основе изопропиламинной соли (глифосата) и ведется поиск маркеров состояния здоровья, изменяющихся под воздействием этих веществ. В качестве маркеров окислительного стресса, вызванного гербицидным составом глифосата, могут использоваться карбонильные производные белков.

Целью данного исследования явилась оценка выраженности окислительного карбонилирования белков ткани печени под воздействием пестицида на основе глифосата в субхроническом эксперименте.

Материал и методы

Материал и методы. Материалом для исследования явились субклеточные фракции гомогенатов ткани печени, полученные от 90 крыс Вистар, которым в течение 3 мес перорально вводили растворы глифосата в дозах 100 и 280 мг/кг. Выраженность окислительного карбонилирования белков определяли по методу R.L. Levine в модификации Е.Е. Дубининой.

Результаты

Результаты. Получены статистически значимые изменения в содержании продуктов окислительного повреждения белков в субклеточных фракциях ткани печени на различных этапах эксперимента в группах исследования по сравнению с показателями контрольной группы.

Заключение

Заключение. Изменения показателей карбонилового стресса и снижение значений показателя резервно-адаптационного потенциала свидетельствуют об истощении антиоксидантной защиты в клетках печени. Статистически значимое нарастание вторичных маркеров карбонилового стресса через 1 мес от начала затравки животных по сравнению с контролем при незначительном повышении этого показателя через 3 мес может свидетельствовать о запуске адаптационных механизмов, в том числе об индуцировании процессов протеолитической утилизации окисленных протеинов или дополнительного синтеза белка.

Introduction

Introduction. Currently, the pathogenetic mechanisms of the action on the body of widely used glyphosate-based herbicides and the search for the markers of the health status that changes under the influence of these herbicides remain open for study and discussion. Carbonyl derivatives of proteins can be used as the markers of oxidative stress caused by the herbicidal composition of the isopropylamine salt of glyphosate.

The purpose of this study was to assess the severity of oxidative carbonylation of liver tissue proteins under the influence of a glyphosate-based pesticide in a subchronic experiment.

Material and methods

Material and methods. The material for the study was subcellular fractions of liver tissue homogenates obtained from 90 Wistar rats, which were administered solutions of glyphosate isopropylamine salt at doses of 280 mg/kg and 100 mg/kg per os for 3 months. The severity of oxidative carbonylation of proteins was determined by the method of R.L. Levine modified by E.E. Dubinina.

Results

Results. Statistically significant changes in the content of products of oxidative damage to proteins at various stages of the experiment in the study groups were obtained compared with the control group due to a significant increase in the products of oxidative damage to proteins in subcellular fractions of liver tissue.

Conclusion

Conclusion. The changes in carbonyl stress indices and the decrease in the reserve-adaptive potential indicate the depletion of antioxidant protection in the liver cells. A statistically significant increase in secondary markers of carbonyl stress after 1 month from the start of seeding of animals compared with the control with a slight increase in this index after 3 months may indicate the launch of adaptation mechanisms, including the induction of proteolytic utilization of oxidized proteins or additional protein synthesis.

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The authors declare that there are no conflicts of interest present.